v-IMG_4893MALDI-TOF MS (matrix assisted laser desorption ionization time-of-flight MS) is a mass spectrometry method that is widely used in protein and glycan research. The new generation MALDI mass spectrometers allow a precise mass determination of proteins, peptides and glycans as well as the fragmentation (=MS/MS) of these to derive protein or peptide sequence data. This increases the confidence in protein or peptide identification significantly.

MALDI-MS is perfectly suited for the detailed structural characterization of proteins, including the analysis of post-translational modifications (PTM) and for the analysis of glycan pools.

For the analysis of the MALDI MS and MS/MS data sets, Protagen Protein Services has developed algorithms that allow getting more information out of the mass spectra.

MALDI-MS can be employed in a wide range of biochemical applications ranging from peptides to proteins and glycans, such as:

  • determining the molecular weight of intact proteins. This can be used to confirm the identity of a protein, screen for protein modifications or to use the molecular weight for the calculation of the mass extinction coefficient or mass concentration.
  • Identifying proteins, e.g. after 1D or 2D gel electrophoresis as well as in the in-depth characterization of proteins and PTMs.
  • MALDI-TOF MS can be used for the proof of identity according to ICHQ6B.

 

The sample is mixed with a matrix, crystallized on a MALDI target and ionized using a UV laser system. The ions are then detected in a time-of-flight analyzer. The data sets are then imported into a relational database for further data reprocessing and evaluation.

MALDI MS is used or can complement a large range of further analysis such as:

As a complementary mass spectrometry approach, Protagen offers liquid chromatography coupled with electrospray ionization MS (LC-ESI-MS and LC-ESI-MS/MS). For N- and C-terminal sequencing of proteins, we recommend the MALDI-ISD technique.

Protagen’s protein analysis package is designed to encompass the requirements of ICH guidelines (in particular ICH Q6B).

 

Your contact for MALDI-TOF Mass Spectrometry in Protein Analytics at Protagen Protein Services GmbH

Marcus-Mreyen

Dr Marcus Mreyen

Director Business Development
Our business development team will be happy to assist you with your project.

Phone: +49 (0) 231 9742-6100
Email: salesproteinservices@ProtagenProteinServices.com

IMG_4703For modern biopharmaceutical research, it is necessary to combine different analytical methods with each other in order to obtain a more complete assessment of the protein structure and function. The analytical methods at Protagen Protein Services GmbH include:

Mass Spectrometry

The application of mass spectrometry in protein research has revolutionized the analysis of the large and complex molecules. Protagen Protein Services GmbH is well equipped to carry out mass spectrometry analyses by MALDI-MS, LC-ESI-MS and MALDI-ISD.

Bio-IT in Mass Spectrometry

The amount of data that is produced by modern mass spectrometers has steadily increased. Completely new algorithms have been developed in order to cope with the increasing data size and complexity. Today, even though many sophisticated algorithms are available on the market, bioinformatics expertise is still needed to fully interpret mass spectrometry data sets.

Liquid Chromatography

High Performance Liquid chromatography (HPLC) encompasses a large range of methods in biopharmaceutical research such as RP-HPLC, IEX-HPLC, SEC-HPLC etc that can be applied in combination with each other as well as in combination with other analytical techniques. This makes HPLC one of the most used analytical techniques in this field, allowing separating any biomolecule from amino acids to protein complexes. The use of UPLC systems has further increased the speed and resolution of these analyses.

Electrophoresis

Electrophoretic methods have been applied to protein research for more than eighty years and allow separating proteins according to different physicochemical parameters such as charge or size. The proteins can be visualized after separation by many staining techniques, such as DIGE, silver stain, colloidal Coommassie etc.

Electrophoresis can be applied to biopharmaceutical research using SDS-PAGE, IEF, 2D PAGE or Western Blotting.

A list of instrumentation can also be found here.

All analysis of the protein, the product-related impurities and process-related impurities can be carried out according to ICH Q6B.

Your contact for Analytical Methods at Protagen Protein Services GmbH

Marcus-Mreyen

Dr Marcus Mreyen

Director Business Development
Our business development team will be happy to assist you with your project.

Phone: +49 (0) 231 9742-6100
Email: salesproteinservices@ProtagenProteinServices.com

IMG_4907LC-ESI-MS is a hyphenated mass spectrometry technique that combines the resolving power of HPLC separation with high mass accuracy of a mass spectrometer. As such, it is useful for the determination of intact protein mass or the in-depth analysis of protein or peptide mixtures. Coupling to a HPLC allows quantifying the detected features either by UV or by the MS signal intensity.

At Protagen Protein Services GmbH, a high-resolution time-of-fight ESI-TOF and a very fast quadrupole ion trap can be used for analysis.

For the analysis of the LC-ESI-MS/MS data sets, Protagen has developed algorithms that allow getting more information out of the mass spectra.

At Protagen Protein Services GmbH, LC-ESI-MS can be employed in a wide range of biochemical applications, such as:

Protagen’s protein analysis package is designed to encompass the requirements of ICH guidelines (in particular ICH Q6B).

Your contact for LC-ESI-MS/MS at Protagen Protein Services GmbH

Marcus-Mreyen

Dr Marcus Mreyen

Director Business Development
Our business development team will be happy to assist you with your project.

Phone: +49 (0) 231 9742-6100
Email: salesproteinservices@ProtagenProteinServices.com

IMG_4883The application of mass spectrometry in protein research has revolutionized the analysis of the large and complex molecules. Different ionization techniques and mass detectors were developed that dramatically has increased the sensitivity, accuracy and resolution of the mass spectrometers in the last 25 years. Also, the mass spectrometers were interfaced with liquid chromatography separation, further expanding the range of applications.

Today, mass spectrometry analysis is an indispensable part of biopharmaceutical research.

At Protagen Protein Services GmbH we apply modern mass spectrometry techniques to the analysis of biomolecules using set of different mass spectrometry techniques:


MALDI-TOF MS (matrix assisted laser desorption ionization time-of-flight MS) is a mass spectrometry method that is widely used in protein and glycan research. The new generation MALDI mass spectrometers allow a precise mass determination of proteins, peptides and glycans as well as the fragmentation (=MS/MS) of these to derive protein or peptide sequence data. This increases the confidence in protein or peptide identification significantly.

LC-ESI-MS is a hyphenated mass spectrometry technique that combines resolving power of HPLC separation with the high mass accuracy of a mass spectrometer. As such, it is useful for the determination of the intact protein mass or the in-depth analysis of protein or peptide mixtures. The coupling to the HPLC allows quantifying the detected features either by UV or by the MS signal intensity. The HPLC can either be interfaced with a ion trap or a q-TOF ESI mass spectrometer.

MALDI-ISD (matrix assisted laser desorption ionization – in source decay) is a mass spectrometry method that is used for the simultaneous sequencing of protein N- and C-termini. The sequencing occurs without cleaving the sample with chemicals or enzymes. In contrast to Edman sequencing, MALDI-ISD will also sequence blocked termini.

For the analysis of mass spectrometry data sets, Protagen has excellent bioinformatics expertise as we have developed algorithms that allow getting more information out of the mass spectra.

Mass spectrometry is increasingly used in the ICHQ6B compliant analysis of biopharmaceuticals.

Your contact for Mass Spectrometry at Protagen Protein Services GmbH

Marcus-Mreyen

Dr Marcus Mreyen

Director Business Development
Our business development team will be happy to assist you with your project.

Phone: +49 (0) 231 9742-6100
Email: salesproteinservices@ProtagenProteinServices.com

IMG_4883MALDI-ISD (matrix assisted laser desorption ionization – in source decay) is a mass spectrometry method that is used for the simultaneous sequencing of protein N- and C-termini. The sequencing occurs without cleaving the sample with chemicals or enzymes. In contrast to Edman sequencing, MALDI-ISD will also sequence blocked termini.

The bioinformatics expertise of Protagen Protein Services GmbH allows interpreting the MALDI-ISD spectra much more reliably and efficiently.

The knowledge about the N- and C-terminus of a protein is important e.g. to confirm that the full-length product has been expressed or to confirm that a signal peptide has been cleaved off.

The intact protein is mixed with a matrix, crystallized on a MALDI target and ionized using a UV laser system. The ions are then detected in a time-of-flight analyzer. The data sets are then imported into a data evaluation software for further data reprocessing.

MALDI ISD can complement a large range of further analysis such as:

Protagen’s protein analysis package is designed to encompass the requirements of ICH guidelines (in particular ICH Q6B).

Your contact for MALDI-ISD at Protagen Protein Services GmbH

Marcus-Mreyen

Dr Marcus Mreyen

Director Business Development
Our business development team will be happy to assist you with your project.

Phone: +49 (0) 231 9742-6100
Email: salesproteinservices@ProtagenProteinServices.com